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KMID : 0545120140240030359
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 3 p.359 ~ p.362
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Enzymatic Production of 15-Hydroxyeicosatetraenoic Acid from Arachidonic Acid by Using Soybean Lipoxygenase
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Kim Baek-Joong
Shin Kyung-Chul
Oh Deok-Kun
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Abstract
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15-Hydroxyeicosatetraenoic acid (HETE), as a mammalian biologically active metabolite, has anticarcinogenic effect. The conditions of producing 15-HETE from arachidonic acid by using soybean lipoxygenase were optimal at pH 8.5 and 20°C with 9 g/l arachidonic acid, 54.4 U/ml soybean lipoxygenase, and 4% methanol. Under these optimized conditions, the enzyme produced 9.5 g/l 15-HETE after 25 min, with a molar conversion yield of 99% and a productivity of 22.8 g l-1 h-1. To the best of our knowledge, this is the first biotechnological production of 15-HETE.
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KEYWORD
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15-hydroxyeicosatetraenoic acid, arachidonic acid, soybean lipoxygenase, enzymatic production
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